Journal: Stem Cells Translational Medicine

Article Title: Detection of residual pluripotent stem cells in cell therapy products utilizing droplet digital PCR: an international multisite evaluation study

doi: 10.1093/stcltm/szae058

Figure Lengend Snippet: Precision of ddPCR assay using the same RNA samples prepared from iPSC-spiked cardiomyocytes and human heart in step 1 study.

Article Snippet: Cryopreserved iCell CM was thawed with iCell Cardiomyocytes Plating Medium (FUJIFILM Cellular Dynamics) according to the manufacturer’s instructions.

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Journal: bioRxiv

Article Title: Ion channel expression and electrophysiology of singular human (primary and induced pluripotent stem cell derived) cardiomyocytes

doi: 10.1101/2021.03.04.433834

Figure Lengend Snippet: Data are shown as mean ± SD. Students t-test was used to compare groups. * indicates p<0.05; ** indicates p<0.01, and *** indicates p<0.001. The numbers of total recordings used to calculate means are listed in . Note that electrophysiological recordings were not available from primary atrial cardiomyocytes.

Article Snippet: For a high percentage of single hiPSC-CMs, spontaneous beating was observed (iCell cardiomyocytes: 83 %, ventricular Pluricytes: 66 %, atrial Pluricytes: 58%).

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Journal: bioRxiv

Article Title: Ion channel expression and electrophysiology of singular human (primary and induced pluripotent stem cell derived) cardiomyocytes

doi: 10.1101/2021.03.04.433834

Figure Lengend Snippet: To display the spectrum of action potential durations, a cell with a long and a cell with a short action potential for each cell group ( (a) iCell cardiomyocytes, (b) ventricular and (c) atrial Pluricytes, (d) primary adult human ventricular cardiomyocytes) was selected. A short action potential is shown in green and a long action potential is shown in red. The numbers of total recordings are listed in . Note that electrophysiological recordings were not available from primary atrial cardiomyocytes.

Article Snippet: For a high percentage of single hiPSC-CMs, spontaneous beating was observed (iCell cardiomyocytes: 83 %, ventricular Pluricytes: 66 %, atrial Pluricytes: 58%).

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Journal: bioRxiv

Article Title: Ion channel expression and electrophysiology of singular human (primary and induced pluripotent stem cell derived) cardiomyocytes

doi: 10.1101/2021.03.04.433834

Figure Lengend Snippet: Evaluation of histograms to confirm cardiac subpopulations. In (a) the separated data sets of primary atrial and ventricular cardiomyocytes clearly show the expected left-shifted distribution of atrial cardiomyocytes (higher expression of this atrial-associated gene). However, the histogram of the pooled data sets in (b) demonstrates that differences between atrial and ventricular primary cardiomyocytes are too small to be resolved (for our number of investigated cells). Thus, it is unlikely that histograms of expression data from iCell cardiomyocytes (c) are suitable to confirm three subpopulations. For total n see . The histograms of the other ion channels investigated can be found in Supp .

Article Snippet: For a high percentage of single hiPSC-CMs, spontaneous beating was observed (iCell cardiomyocytes: 83 %, ventricular Pluricytes: 66 %, atrial Pluricytes: 58%).

Techniques: Expressing

Journal: bioRxiv

Article Title: Ion channel expression and electrophysiology of singular human (primary and induced pluripotent stem cell derived) cardiomyocytes

doi: 10.1101/2021.03.04.433834

Figure Lengend Snippet: As data of electrophysiological parameters are only available for primary ventricular cardiomyocytes (and not for primary atrial cardiomyocytes), we cannot evaluate whether shapes of histograms of electrophysiological parameters mirror cardiac subpopulations. However, the histograms of electrophysiological data from iCell cardiomyoctes do not indicate subpopulations. The total range of values is bigger than for primary ventricular cardiomyocytes (except for capacitance). For total n see .

Article Snippet: For a high percentage of single hiPSC-CMs, spontaneous beating was observed (iCell cardiomyocytes: 83 %, ventricular Pluricytes: 66 %, atrial Pluricytes: 58%).

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Journal: bioRxiv

Article Title: High-throughput optical sensing of peri-cellular oxygen in cardiac cells: system characterization, calibration, and testing

doi: 10.1101/2023.04.24.538133

Figure Lengend Snippet: A. Oxygen readout (ratio values) spread across the 96-well plate as exposure time varies between 0.15 to 1.5 s. To minimize the spread, exposure was chosen in the 0.4 to 0.9 s range. B. Spatial variation across 96-well plate with 0% oxygen (Cal0), blue, and air saturated (Cal100) oxygen for culture medium used to grow human cardiac myocytes, CM (top), and human cardiac fibroblasts, cFB (bottom). 5% Na 2 SO 3 in the medium was used for 0% oxygen calibration; exposure time set to 0.8 sec.

Article Snippet: Two types of culture media, CDI iCell Cardiomyocytes maintenance medium (Fujifilm CDI) and cardiac fibroblasts growth medium (Cell Applications, Inc), were used.

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